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Mass Spectrometry Facility

The Mass Spectrometry Facility provides analytical support for the Chemistry Department and other academic departments at LSU, researchers at other universities, and customers from private industry. The facility offers a broad range of services with several high performance mass spectrometers (listed below). This facility is staffed by Dr. Azeem Hasan (director), Dr. Dan Pu (research associate), and supported by graduate research assistants. Instruments are located in rooms B-9 and B-24 Choppin Hall (phone 225-578-3575).

 

Sample Submission

 

Small molecule sample submission form

    Use this form for small organic or inorganic molecules or non-biological macromolecules.

 

Biomolecule sample submission form

Use this form for biomolecules and proteomics analysis.

 

Mass Spectrometers


InstrumentIonization ModeTechnique(s)Analytes
Varian Saturn 2200

Electron ionization (EI)

Chemical ionization (CI)

Gas chromatography/mass spectrometry (GC/MS)

Volatile organic molecules

Complex mixtures

Agilent 6210

Electrospray ionization (ESI)

Atmospheric pressure chemical ionization (APCI)

Exact mass determination

Liquid chromatography mass spectrometry

Organic and inorganic molecules

Peptides and proteins

Applied Biosystems QSTAR

Electrospray ionization (ESI)

Nanoelectrospray

Atmospheric pressure chemical ionization

 

Liquid chromatography mass spectrometry

Multidimensional liquid chromatography (e.g. MudPIT)

Peptide sequence tags

De novo sequencing

Isotopic labeling (iCAT, ITRAQ, etc.)

Peptides

Proteins

Oligonucleotides

Bruker OmniflexMatrix-assisted laser desorption ionization (MALDI)

Intact mass

Peptide mass map

Peptides

Proteins

Oligonucleotides

Synthetic polymers

Organics

Some inorganics

Bruker Ultraflex ExtremeMatrix-assisted laser desorption ionization (MALDI)

Intact mass

Peptide mass map

Peptide sequence tag

Top down sequencing

Liquid chromatography mass spectrometry (off-line)

Tissue imaging

Peptides

Proteins

Oligonucleotides

Synthetic polymers

Organics

Some inorganics

Mascot Computer ClusterN/ADatabase searchProteins

 

 

Rates

 

Rates are hourly with a one hour minimum per sample (unless user run). The Varian Saturn and Bruker Omniflex are available to trained users for hourly work. Please contact Dr. Hasan for information on user training and certification.

 

InstrumentTechniqueLSU CampusOutside LSU AcademicIndustry
Varian Saturn 2200 GCMS$8$11$40
GCMS (User Run)6NANA
Bruker Omniflex MALDI1824100
MALDI (User Run)12NANA
Agilent 6210   ESI Accurate Mass1520100
ESI AM (User Run)10NANA
LCMS2533100
LCMS (User Run)20NANA
Applied Biosystems QSTAR          ESI MS1520100
Exact Mass5060100
Capillary LCMS2560100
Nano LCMS or MS/MS4053100
Nano LCMS (Own Column)2533100
2D Gel MALDI and ESI MS/MS (per spot)4053100
2D LC MS/MS (per step)30 100
Proteomics Sample Prep2533100
Database Search2027100
oMALDI4053100
iTRAQ40+kit cost53+kit cost100+kit cost
 

 

Faculty Contact

 

Prof. Kermit Murray

225-578-3417

Choppin 331

 

Dr. Kermit Murray is the primary faculty contact for the Mass Spectrometry Facility. He joined the LSU faculty in 2001 after seven years on the faculty of Emory University. Dr. Murray has more than 25 years of experience in mass spectrometry and ion chemistry. He received his Ph.D. from the University of Colorado and went on to post doctoral positions at Rice University and at Texas A&M University. Dr. Murray’s research is in the area of instrument development and applications of biological and environmental mass spectrometry.

 

Staff Scientists

 

Dr. Azeem Hasan
225-578-3389
Choppin B-16

 

Dr. Azeem Hasan, the Mass Spectrometry Facility Director, received his Ph.D. in 1995 from the University of Karachi, Pakistan. He joined the staff of the facility in May, 2006 and has more than 15 years of experience in the field of mass spectrometry. Prior to moving to LSU, Dr. Hasan was manager of the MS facility at the University of New Mexico (UNM). At UNM and other appointments, Dr. Hasan has experience in a wide variety of analytes ranging from small molecules to complex macromolecules including intact proteins, protein digests, oligonucleotides, carbohydrates, pesticides, human metabolites, and natural products.

 

Dr. Dan Pu
225-578-8547
Choppin B-24

 

Dr. Dan Pu received her Ph.D. in 2007 from the University of Alabama, Tuscaloosa under the direction of Dr. Carolyn Cassady (although she is now an avid LSU Tiger fan). She joined the LSU Mass Spectrometry Facility in September 2007. Dr. Pu has extensive experience with peptides analysis by mass spectrometers. Her Ph.D. work was aimed at exploring the coordination of Cr-bound chromodulin and investigating the fragmentation of peptides containing amino acid residues with basic side chains and amino acid residues containing hydroxyl side chains.  Prior to her Ph.D. studies in USA, she acquired her Master degree in Beijing Mass Spectrometry Center in the Institute of Chemistry, Chinese Academy of Sciences. She has extensive experience with the analysis of a wide range of compounds ranging from small synthetic molecules to large macromolecules including peptides, carbohydrates, and intact proteins.

 

 

 

Links

 

Murray Group Server

Cyber Mass Spectrometry Server