The Mass Spectrometry Facility provides analytical support for the Chemistry Department and other academic departments at LSU, researchers at other universities, and customers from private industry. The facility offers a broad range of services with several high performance mass spectrometers (listed below). This facility is staffed by Dr. Azeem Hasan (director), Dr. Dan Pu (research associate), and supported by graduate research assistants. Instruments are located in rooms B-9 and B-24 Choppin Hall (phone 225-578-3575).
Sample Submission
• Small molecule sample submission form
Use this form for small organic or inorganic molecules or non-biological macromolecules.
• Biomolecule sample submission form
Use this form for biomolecules and proteomics analysis.
Mass Spectrometers
| Instrument | Ionization Mode | Technique(s) | Analytes |
| Varian Saturn 2200 | Electron ionization (EI) Chemical ionization (CI) | Gas chromatography/mass spectrometry (GC/MS) | Volatile organic molecules Complex mixtures |
| Agilent 6210 | Electrospray ionization (ESI) Atmospheric pressure chemical ionization (APCI) | Exact mass determination Liquid chromatography mass spectrometry | Organic and inorganic molecules Peptides and proteins |
| Applied Biosystems QSTAR | Electrospray ionization (ESI) Nanoelectrospray Atmospheric pressure chemical ionization
| Liquid chromatography mass spectrometry Multidimensional liquid chromatography (e.g. MudPIT) Peptide sequence tags De novo sequencing Isotopic labeling (iCAT, ITRAQ, etc.) | Peptides Proteins Oligonucleotides |
| Bruker Omniflex | Matrix-assisted laser desorption ionization (MALDI) | Intact mass Peptide mass map | Peptides Proteins Oligonucleotides Synthetic polymers Organics Some inorganics |
| Bruker Ultraflex Extreme | Matrix-assisted laser desorption ionization (MALDI) | Intact mass Peptide mass map Peptide sequence tag Top down sequencing Liquid chromatography mass spectrometry (off-line) Tissue imaging | Peptides Proteins Oligonucleotides Synthetic polymers Organics Some inorganics |
| Mascot Computer Cluster | N/A | Database search | Proteins |
Rates
Rates are hourly with a one hour minimum per sample (unless user run). The Varian Saturn and Bruker Omniflex are available to trained users for hourly work. Please contact Dr. Hasan for information on user training and certification.
| Instrument | Technique | LSU Campus | Outside LSU Academic | Industry |
| Varian Saturn 2200 | GCMS | $8 | $11 | $40 |
| GCMS (User Run) | 6 | NA | NA | |
| Bruker Omniflex | MALDI | 18 | 24 | 100 |
| MALDI (User Run) | 12 | NA | NA | |
| Agilent 6210 | ESI Accurate Mass | 15 | 20 | 100 |
| ESI AM (User Run) | 10 | NA | NA | |
| LCMS | 25 | 33 | 100 | |
| LCMS (User Run) | 20 | NA | NA | |
| Applied Biosystems QSTAR | ESI MS | 15 | 20 | 100 |
| Exact Mass | 50 | 60 | 100 | |
| Capillary LCMS | 25 | 60 | 100 | |
| Nano LCMS or MS/MS | 40 | 53 | 100 | |
| Nano LCMS (Own Column) | 25 | 33 | 100 | |
| 2D Gel MALDI and ESI MS/MS (per spot) | 40 | 53 | 100 | |
| 2D LC MS/MS (per step) | 30 | 100 | ||
| Proteomics Sample Prep | 25 | 33 | 100 | |
| Database Search | 20 | 27 | 100 | |
| oMALDI | 40 | 53 | 100 | |
| iTRAQ | 40+kit cost | 53+kit cost | 100+kit cost |
Faculty Contact
225-578-3417
Choppin 331
Dr. Kermit Murray is the primary faculty contact for the Mass Spectrometry Facility. He joined the LSU faculty in 2001 after seven years on the faculty of Emory University. Dr. Murray has more than 25 years of experience in mass spectrometry and ion chemistry. He received his Ph.D. from the University of Colorado and went on to post doctoral positions at Rice University and at Texas A&M University. Dr. Murray’s research is in the area of instrument development and applications of biological and environmental mass spectrometry.
Staff Scientists
Dr. Azeem Hasan
225-578-3389
Choppin B-16
Dr. Azeem Hasan, the Mass Spectrometry Facility Director, received his Ph.D. in 1995 from the University of Karachi, Pakistan. He joined the staff of the facility in May, 2006 and has more than 15 years of experience in the field of mass spectrometry. Prior to moving to LSU, Dr. Hasan was manager of the MS facility at the University of New Mexico (UNM). At UNM and other appointments, Dr. Hasan has experience in a wide variety of analytes ranging from small molecules to complex macromolecules including intact proteins, protein digests, oligonucleotides, carbohydrates, pesticides, human metabolites, and natural products.
Dr. Dan Pu
225-578-8547
Choppin B-24
Dr. Dan Pu received her Ph.D. in 2007 from the University of Alabama, Tuscaloosa under the direction of Dr. Carolyn Cassady (although she is now an avid LSU Tiger fan). She joined the LSU Mass Spectrometry Facility in September 2007. Dr. Pu has extensive experience with peptides analysis by mass spectrometers. Her Ph.D. work was aimed at exploring the coordination of Cr-bound chromodulin and investigating the fragmentation of peptides containing amino acid residues with basic side chains and amino acid residues containing hydroxyl side chains. Prior to her Ph.D. studies in USA, she acquired her Master degree in Beijing Mass Spectrometry Center in the Institute of Chemistry, Chinese Academy of Sciences. She has extensive experience with the analysis of a wide range of compounds ranging from small synthetic molecules to large macromolecules including peptides, carbohydrates, and intact proteins.
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